Fig. 7: Diet induced differential expression of neuroinflammatory genes in 3xTg-AD mice. | Nature Communications

Fig. 7: Diet induced differential expression of neuroinflammatory genes in 3xTg-AD mice.

From: Fasting is required for many of the benefits of calorie restriction in the 3xTg mouse model of Alzheimer’s disease

Fig. 7

A–D Expression of the indicated pro-inflammatory and anti-inflammatory genes from female (A, B) and male (C, D) 3xTg mice fed on ad libitum (AL), Diluted AL (DL) or Caloric Restricted (CR with fasting between once-daily meals) diets from 6–15 months of age using qPCR. E Summary of statistical outcomes from two-way ANOVAs (Diet x Sex) conducted separately for each gene. A, B n = 5 AL, n = 5 DL, n = 6 CR fed 3xTg biologically independent female mice (C, D) n = 5 AL, n = 5 DL, n = 5 CR fed 3xTg biologically independent male mice. A–D Statistics for the overall effect of diet (D), genes (G), and the interaction represent the p values from a two-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Sidak’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. E Statistics for the overall effect of diet (D), sex (S), and the interaction represent the p values from a two-way ANOVA. Data represented as mean ± SEM. Source data are provided as a Source Data file.

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