Fig. 8: Differential effects of feeding regimens on brain mTORC1 signaling and autophagy activation in 3xTg mice.

A–L The phosphorylation of S6 and 4E-BP1, and the expression of p62, LC3A/B and BDNF was assessed by western blotting of whole brain lysates of 3xTg mice fed on ad libitum (AL), Diluted AL (DL) or Caloric Restricted (CR with fasting between once-daily meals) diets from 6-15 months. Quantification of the phosphorylation of p-S240/S244 S6 (B) and T37/S46 4E-BP1 (C), relative to expression of S6 and 4E-BP1, respectively in females. Quantification of p62 expression (D) LC3A/B expression (E) and BDNF expression (F) relative to expression of GAPDH in females. Quantification of the phosphorylation of p-S240/S244 S6 (H) and T37/S46 4E-BP1 (I), relative to expression of S6 and 4E-BP1, respectively, in males. Quantification of p62 expression (J), LC3A/B expression (K), and BDNF expression (L) relative to expression of GAPDH in males. (B–F) n = 4 AL, n = 5 DL and n = 5 CR fed 3xTg biologically independent female mice. H n = 4 AL, n = 4 DL and n = 4 CR (I) n = 4 AL, n = 5 DL and n = 4 CR (J, K) n = 4 AL, n = 5 DL and n = 5 CR (L) n = 4 AL, n = 5 DL and n = 4 CR fed 3xTg biologically independent male mice. B–F, H–L Statistics for the overall effects of diet (D), sex (S) and their interaction (DxS) represent the p values from a 2-way ANOVA; *p < 0.05, from a two-tailed Tukey’s test post ANOVA. Data represented as mean ± SEM. BDNF; Brain derived neurotrophic factor. Source data are provided as a Source Data file.