Fig. 1: Analyses of fibrotic parameters in BLM-injured lungs from young and old mice.

A Experimental design. Young (7 weeks) or old (18 months) mice were treated either with BLM or PBS. Their lungs were collected at the BLM-induced fibrotic peak at day 14 and during lung regeneration (day 28) and fibrosis resolution (day 60). B Hydroxyproline quantification on BLM (n ≥ 6) or PBS (n = 4)-treated young or old mouse samples at indicated time points. Source data are provided as a Source Data file. Boxplot are represented with the median in the center, the whiskers correspond to the interquartile ranges, and the bounds correspond to the minimum and maximum values. * P < 0.05. **P < 0.01. P values were calculated by two-way ANOVA test followed by a multiple comparisons test with Holm-Šídák correction. C Histological sections of control and fibrotic lungs from young and old mice using H&E at indicated time points (representative images, n = 3). D Experimental design of spatial transcriptomics data from histological sections (H&E) of lungs from young (n = 1) and old (n = 2) mice challenged with BLM and collected at fibrotic peak (day 14, D14) and during regeneration (day 28, D28). Spots captured corresponding to tissue area were deconvoluted through ST deconvolve package in topics (n = 14). E Functional annotations of topics by IPA. Enrichment p-values obtained with IPA are calculated by right-tailed Fisher’s Exact Test and Benjamini-Hochberg correction. F Average proportion of each topic across deconvoluted spots in each slice. A, D created in BioRender. MARI, B. (2025) https://BioRender.com/b0jq8np.