Fig. 4: Lrg1pos PCEC dynamics are delayed in old mice.
From: Aging affects reprogramming of pulmonary capillary endothelial cells after lung injury in male mice
A Pulmonary endothelial subpopulations relative frequencies in young and old mice across time points. B BLM-induced signature score (see material and methods section) in gCap (top) and aCap (bottom) in young and old mice across time points. C In situ hybridization of Col15a1 mRNA at indicated time points in young and old mice. Scale bars = 20 µm. D RNA FISH of Lrg1 mRNA with gCap marker Aplnr mRNA or aCap marker Ednrb mRNA at indicated time points in young and old mice. (n = 3 independent mice, 3 counted microscopic fields/mouse, scale bars = 20 µm). Quantification was performed in three different fields for each mouse. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. P-values were calculated by a Two-way ANOVA test followed by a multiple comparisons test with Holm-Sidak correction. Boxplot are represented with the median in the center, the whiskers correspond to the interquartile ranges, and the bounds correspond to the minimum and maximum values. Source data are provided as a Source Data file.
