Fig. 5: Aged mouse lung gCap exhibits pro-inflammatory and injury-activated phenotype.

A Differentially expressed genes (DEGs) between old and young gCap in fibrotic condition. B Expression of several genes dysregulated in age-related in gCap under BLM conditions (Cd74, Gbp4, H2-Ab1, Klf2, Klf10 and Peg3). Boxplot of relative expression, each point corresponds to one mouse (n = 3 by condition, ● for D14 ▲ for D28). C In situ hybridization of Cd74 mRNA in BLM or Day 14 after BLM induction in young and old mice. Scale bars = 20 µm. D DEGs between old and young gCap in physiological condition. E Pseudobulk expression of several genes dysregulated by ageing in gCap under PBS conditions (Aplnr, Col15a1, Lrg1, Slc6a2, Ntrk2, Plat, Prss23 and Vwf). Each point corresponds to the aggregated expression in one mouse (n = 3 by condition, ● for D14, ▲ for D28 and ■ for D60). F Function enrichment analysis on DEGs between old and young physiological gCap. G Comparison of the log₂FC obtained by comparing physiological gCap of old and young mice, and the corresponding log₂FC between gCap of BLM and PBS-treated mice. Boxplot are represented with the median in the center, the whiskers correspond to the interquartile ranges, and the bounds correspond to the minimum and maximum values. Source data are provided as a Source Data file. Statistic: P-values were calculated by the Wald test and the Benjamini-Hochberg method for multiple tests correction.