Fig. 3: Ion exclusion facilitates homotypic protein phase separation at low salt conditions.
From: Quantifying collective interactions in biomolecular phase separation
a Phase separation of FUS-EGFP was studied in the presence of potassium chloride (KCl). b Dilute phase concentration changes of FUS at varying total KCl concentrations. c Determination of the FUS/KCl phase boundary from dilute phase concentration changes in (a). d Schematic representation of the dominance value of FUS. e Dense phase EGFP fluorescent lifetimes (tden) at 2 µM FUS and varying KCl concentrations. Data are presented as mean ± SD from n > 3 repeats. Dilute phase concentration changes of proteins TDP43 (f) and PGL3 (g) at varying total KCl concentrations. Grey dotted lines highlight the FUS saturation concentration for comparison. h Schematic illustration of ion behaviour for FUS phase separation at low KCl concentrations. Source data are provided as a Source Data file.
