Fig. 4: Ions are enriched in FUS condensates under high salt reentrant conditions to foster non-ionic interactions.
From: Quantifying collective interactions in biomolecular phase separation
a FUS displays reentrant phase separation at high salt concentrations. b FUS dilute phase line scans at varying total LiCl (green) and CsCl (blue) concentrations. c Phase boundaries show that phase separation occurs only above the respective critical salt and FUS concentrations. d Comparison of ion partition (1/K) of LiCl and CsCl into FUS condensates. Preferential partitioning at high ionic strength drives enhancement of non-ionic interactions. Data are presented as mean ± SD, parameter errors are estimated by repeated perturbation on fitting data and quantifying the spread of best-fit parameters. e High salt FUS phase separation indicates extreme cases where phase separation is driven by only the limiting component. This leads to dominance values of 0 or 1 depending on the position on the phase boundary. Source data are provided as a Source Data file.
