Fig. 5: 1,6-hexanediol (1,6-HD) decreases the phase separation propensity by modulating solvent quality.
From: Quantifying collective interactions in biomolecular phase separation
a To characterise the mechanism of action of 1,6-hexanediol, phase separation of FUS with PEG was studied in the presence (+) and absence (−) of the compound. b FUS dilute phase concentration changes with varying concentrations of polyethylene glycol (PEG) in the presence (+, green) and absence (−, blue) of 1,6-HD (7% (w/v)). c Phase boundaries for FUS against PEG in the absence and presence of 1,6-HD were created from changes in dilute phase concentrations and tie line gradients. d (left) Shift in PEG partitioning (1/K) in the absence and presence of 1,6-HD, indicating a change in condensate stoichiometry by decreasing PEG/protein ratio. (right) FUS dominance in the presence and absence of 1,6-HD. Data are presented as mean ± SD, parameter errors are estimated by repeated perturbation on fitting data and quantifying the spread of best-fit parameters. e Changes in hydrodynamic radius Rh as a function of 1,6-HD concentration. Shown are data for monomeric FUS-EGFP (dark green) and EGFP protein alone (light green) at 1 µM protein concentration each. Data are presented as mean ± SD from n = 3 repeats. f Change in hydrodynamic radius for a set of proteins with increasing 1,6-HD concentration. Data are presented as mean ± SD from n = 3 repeats. Source data are provided as a Source Data file.
