Fig. 4: Sustained overexpression of 12,13-diHOME improves cardiac function and remodeling in aged mice. | Nature Communications

Fig. 4: Sustained overexpression of 12,13-diHOME improves cardiac function and remodeling in aged mice.

From: 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII

Fig. 4

a Schematic illustration of tissue nano-transfection (TNT)- epoxide hydrolase 1 and 2 (Ephx1/2) intervention (Created in BioRender. Baer, L. (2025) https://BioRender.com/0c6k2kp). b Volcano plot of signaling lipids, c quantification of 12,13-diHOME; n = 12 (sham) and n = 15 (TNT), and d TNT-Ephx1/2 increased gene expression of Ephx1 and 2 in the skin; n = 7 (sham) and n = 6 (TNT). e Ejection fraction, f left ventricular posterior wall thickness (LVPW), g hypertrophy index; n = 13 (sham males), n = 7 (TNT males), n = 10 (sham females), n = 8 (TNT females), h systolic function, and i diastolic function in aged mice; n = 6 (sham males), n = 7 (TNT males), n = 9 (sham females), n = 5 (TNT females). Data are expressed as mean ± SEM. Volcano plot shows the log2 fold change versus –log10 (p) value from two-sided Student’s ttests. False discovery rate (FDR) correction was applied, with significance threshold set at FDR < 0.05 (b). Two-tailed P values by unpaired Student’s ttest (c–i). Bar colors: white (sham), purple (TNT) (c, d). Bar colors: white (sham), blue (TNT males), pink (TNT females) (e, i).

Back to article page