Fig. 3: Aau and Rbl mediate D-ribulose transport in both EHEC and C. rodentium.
From: Convergent evolution of distinct D-ribulose utilisation pathways in attaching and effacing pathogens
a Schematic illustration of the similar roles for Aau and Rbl in D-ribulose transport in both species. b Growth analysis of wild type EHEC in M9 minimal media supplemented with D-ribulose as a sole carbon source. c Growth analysis of EHEC ∆aau cross-complemented with the entire Rbl system (pSU-rbl) from C. rodentium in M9 plus D-ribulose. d Growth analysis of wild type C. rodentium in M9 plus D-ribulose. e Growth analysis of C. rodentium ∆rblABCD (ROD_24811-41; ABC transporter components only) in M9 plus D-ribulose. f Growth analysis of C. rodentium ∆rblABCD (ROD_24811-41) in M9 plus D-ribulose cross-complemented with the Aau system (pSU-aau) from EHEC. For all experiments illustrated in b, f, M9 minimal media were supplemented with 0.5 mg/mL D-ribulose (blue). The no sugar control (black) indicates the respective strain inoculated into M9 without a carbon source. All growth experiments were performed on three independent occasions (n = 3), and the error bars represent standard deviation from the mean. Source data are provided as a Source Data file.
