Fig. 2: The effect of force on TOP2α resolution.

a Mean resolution probability of TOP2α-AF555 acting on DNA held at different forces, modelled as a Bernoulli process, with 5-minute sampling time. Number of resolution events out of total number of technical replicates is indicated above each force condition (5, 15, 25, 30 and 45 pN); error bars indicate one standard error. Source data are provided as a Source Data file. b Example images from failed resolution event at 25 and 45 pN. c Resolution time after entering the protein channel at different mean forces. N refers to the number of molecules tested in different technical replicates. Box plot whiskers represent the full data range (minimum to maximum), boxes indicate the interquartile range (25th to 75th percentiles), and the central line marks the median. Source data are provided as a Source Data file. d Estimated crossing angle, α, at different mean forces (5, 15, 25, 30 and 45 pN). The total number of technical replicates per force was as in (a) (5 pN: n = 12, 15 pN: n = 12, 25 pN: n = 11, 30 pN: n = 10 and 45 pN: n = 10, pN). Error bars indicate standard error. Source data are provided as a Source Data file. e Schematic depicting crossing angle, α, between the two braided DNAs.