Table 2 Oligonucleotide sequences used in the experiments
Oligo no | Name | Sequence 5′ to 3′ |
|---|---|---|
1 | Hairpin A1 | CCCAAACCCAATTAATGTACTGCAGAATTCAGCTCGAAGCTTGGCCGGATCCGTGAGCTGTCGTC |
2 | Hairpin A2 | /5Phos/TCAGATCGGATACGGCGACCACCGAGATCTACACCCTGCGGGACACTCTTTCCCTACACGACGCTCTTCCGATCTGAGACGACAGCTCAC |
3 | Hairpin A3 | CCGGCCAAGCTTCGAGCTGAATTCTGCAGTACATTAATTGGGTTTGGG |
4 | Hairpin S1 | CCCAAACCCAATTAATGTACTGCAGAATTCAGCTCGAAGCTTGGCCGGATCCAGCGTGGGACTGAGTC |
5 | Hairpin S2 | /5Phos/GTCTCGTGTCTGTAAAAACGTACGTAGATGCCATTTCTAAAAAAACAGACACGAGACGACTCAGTCCCACGCT |
6 | Hairpin S3 | CCGGCCAAGCTTCGAGCTGAATTCTGCAGTACATTAATTGGGTTTGGG |
7 | Adapter S1 | /5Phos/CGCACTGAGACTGATATGTGAAAAATTAGATTGGATAACTGCGCAGAAAAACACATATCAGTCTCAGTGCG |
8 | Fwd primer S1 | CTGCGCAGTTATCCAATCTAA |
9 | Rev primer S1 | CGTACGTAGATGCCATTTCTA |
10 | Fwd primer S2 | GTAAAACGACGGCCAG |
11 | Rev primer S2 | CAGGAAACAGCTATGAC |
12 | Hairpin (sequencing) | GCAACCAGACAGGTATGAACGCGCGCTACTGTTTTCAGCTCGAAGCTTGGCTGTGGAGACCGTGGGACTGAGTC |
13 | Hairpin (sequencing) | /5Phos/GGATCCCGTGTCTGTCATGTTACAGACACGGGATCCGACTCAGTCCCACGGTCTC |
14 | Hairpin (sequencing) | CAGCCAAGCTTCGAGCTGAAAACAGTAGCGCGCGTTCATACCTGTCTGGTTG/3ddC/ |
15 | Adapter i5-1F | /5Phos/AATGATACGGCGACCACCGAGATCTACACAGCGCTAGACACTCTTTCCCTACACGACGCTCTTCCGATCT |
16 | Adapter i5-1R | /5Phos/GATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT/3ddC/ |
17 | Adapter i7-1F | /5Phos/GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCGGTTATCTCGTATGCCGTCTTCTGCTTG |
18 | Adapter i7-1R | /5Phos/GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
19 | Adapter i5-2F | /5Phos/AATGATACGGCGACCACCGAGATCTACACCGCAGACGACACTCTTTCCCTACACGACGCTCTTCCGATCT |
20 | Adapter i7-2F | /5Phos/GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTATGCCGTCTTCTGCTTG |
21 | Primer FWD | AATGATACGGCGACCACCGA |
22 | Primer REV | CAAGCAGAAGACGGCATACG |
23 | Adapter I3 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
24 | Adapter I4 | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG |
25 | Adapter I5 | CTGTCTCTTATACACATCT |
26 | Fwd primer I1 | AATGATACGGCGACCACCGAGATCTACACCCTGCGGGTCGTCGGCAGCGTCAGATGTGTAT |
27 | Rev primer I1 | CAAGCAGAAGACGGCATACGAGATCTGTATTTGTCTCGTGGGCTCGGAGATGTG |
28 | Rev primer I2 | CAAGCAGAAGACGGCATACGAGATGACCCAAGGTCTCGTGGGCTCGGAGATGTG |
29 | Rev primer I3 | CAAGCAGAAGACGGCATACGAGATTTTAACGCGTCTCGTGGGCTCGGAGATGTG |
30 | CHD4 FW | CTGTTGCTGACTGGGACACCAT |
31 | CHD4 Rev | TGGTCCTCCTTGGCAATGTCAG |
32 | GAPDH FW | GGAGTCAACGGATTTGGTCGTA |
33 | GAPDH Rev | GGCAACAATATCCACTTTACCA |
34 | IL-11 FW | CGAGCGGACCTACTGTCCTA |
35 | IL-11 Rev | GCCCAGTCAAGTGTCAGGTG |
36 | JUNB FW | ACTCATACACAGCTACGGGATACG |
37 | JUNB Rev | GGCTCGGTTTCAGGAGTTT |
38 | SERPINE1 FW | GAGACAGGCAGCTCGGATTC |
39 | SERPINE1 Rev | GGCCTCCCAAAGTGCATTAC |
40 | SMAD7 FW | ACCCGATGGATTTTCTCAAACC |
41 | SMAD7 Rev | GCCAGATAATTCGTTCCCCCT |
42 | Adapter P1 | /5Phos/AATTGTTCCCTACACGGACTGAATACTCTGGCCGTCGTTTTAC |
43 | Adapter P2 | /5Phos/GATCGTTCCCTACACGGACTGAATACTCTGGCCGTCGTTTTAC |
44 | Adapter P3 | /5Phos/CATGCTTCCCTACACGGACTGAATACTCTGGCCGTCGTTTTAC |
45 | Adapter P4 | /5Phos/AGCTGTTCCCTACACGGACTGAATACTCTGGCCGTCGTTTTAC |
46 | Adapter P5 | /5Phos/CTTCCCTACACGGACTGAATACTCTGGCCGTCGTTTTAC |
47 | Adapter P6 | CAGGAAACAGCTATGACAGTATTCAGTCCGTGTAGGGAAC |
48 | Fwd primer P1 | GTAAAACGACGGCCAG |
49 | Rev primer P1 | CAGGAAACAGCTATGAC |
