Fig. 5: Mutation of HS/protein receptor binding sites diminishes protection conferred by a receptor decoy inhibitor. | Nature Communications

Fig. 5: Mutation of HS/protein receptor binding sites diminishes protection conferred by a receptor decoy inhibitor.

From: Three positively charged binding sites on the eastern equine encephalitis virus E2 glycoprotein coordinate heparan sulfate- and protein receptor-dependent infection

Fig. 5

Survival data for mice treated intraperitoneally with 100 µg of VLDLR LA(1-2)-Fc (dashed line) or LDLRAD3 LA1-Fc (solid line) as a negative control. Six hours after treatment, mice were infected fp. with equal genome copies of WT and mutant EEEV viruses equivalent to 103 WT PFU (n = 6 mice from 2 independent experiments for LDLRAD3 LA1-Fc and n = 8 mice from 2 independent experiments for VLDLR LA(1-2)-Fc). A EEEV WT (FL93-939). B EEEV 71–77. C EEEV 84–119. D EEEV 156–157. Significance of survival for mice treated with control versus VLDLR LA(1-2)-Fc was determined by log-rank test. Exact p-values are indicated. Source data provided as a Source Data file.

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