Fig. 3: Example CA1 recording session.

a Schematic drawing of the imaging conditions, including the titanium cranial window implant and the objective lens from the UCLA 2P Miniscope. b 1P image collected in the same animal, in the same brain region on the same day as all other panels except E. 1P Image was collected with a custom-made benchtop 1P microscope with a 0.5 NA and high resolution scientific Complementary Metal Oxide Semiconductor (sCMOS) image sensor. c Experimental mouse in the behavioral chamber, during the >20-min imaging session. Light-blue line shows a subset of the animal trajectory in the chamber over time. d Imaging results from the microscope system over the course of the free behavior experiment. e Extracted footprints of the active neurons within the FOV (randomly-colored) following motion correction, overlayed on the maximum intensity projection image. f Neuropil subtracted activity from the neurons in (g, h). g Firing locations of a subset of neurons from (f, h) plotted within the behavioral arena. h 25 example statistically significant place cells (single sided, multiple comparison corrected, based on Shannon Information content³⁶).