Fig. 4: Dendritic imaging in cortex during free behavior.
From: Open-source, high performance miniature 2-photon microscopy systems for freely behaving animals
a Schematic drawing of the experimental preparation used to record from RSC and the curvature of the cortical column that makes resolving long dendrites in the cortex possible. b Time-course of still frames over the course of the 30-min recording, highlighting the axial stability of the system and ability to reliably track single projections over substantial timeframes. Axial stability is assessed in more detail within Supplementary Fig. 6. c Calcium signals from somatic (blue) and dendritic ROIs (red) plotted separately. d Aligned and averaged calcium events from dendritic and somatic ROIs. e Pseudo-colored image exported from Suite2P which colors ROIs by aspect ratio. This is superimposed on the mean of the entire recording. f Overlay of mean somatic and dendritic calcium dynamics from a single calcium event. g Animal trajectory over the 30-min experiment.
