Table 1 Summary of FLEP-seq sequencing libraries

From: Pre-mRNA processing factors differentially impact coordination between co-transcriptional cleavage and transcription termination

Sample	Replicate	Nanopore raw reads	Mapped reads	Full-length readthrough	5’ Cleavage products	3’ Cleavage products	Poly(A) Transcripts	Genes mapped	Genes analyzed
WT (seedling)	Rep1	14,929,183	14,458,259	279,671	204,069	39,801	2,778,740	26,173	7065
WT (seedling)	Rep2	16,483,400	14,552,517	183,214	177,349	41,811	1,774,192	25,876	5618
fy	Rep1	9,461,769	9,116,899	219,441	141,731	28,511	1,623,636	23,912	5397
fy	Rep2	7,019,718	6,396,975	132,414	102,304	20,946	1,179,519	23,857	3576
*cpsf30*	Rep1	15,392,032	14,816,762	301,451	249,441	88,298	1,380,226	26,915	7769
*cpsf30*	Rep2	12,304,249	9,820,393	165,706	150,152	27,880	1,806,537	24,710	4846
*fip1*	Rep1	14,061,240	13,067,385	320,128	229,377	42,755	2,714,835	25,216	8059
*fip1*	Rep2	10,116,056	8,885,421	96,988	106,764	32,535	1,699,177	24,572	2491
*fca*	Rep1	12,097,079	11,633,183	189,006	180,740	29,606	2,690,351	25,644	6084
*fca*	Rep2	8,867,460	7,836,776	111,469	115,202	19,401	1,275,622	24,209	2970
*fpa*	Rep1	14,067,623	12,548,565	183,163	146,057	42,438	1,760,466	31,380	5141
*fpa*	Rep2	9,786,999	8,737,036	131,540	121,572	19,341	2,077,755	24,557	3364
*paps1*	Rep1	9,650,484	8,924,526	259,345	187,249	43,466	1,822,160	29,522	6421
*paps1*	Rep2	7,488,820	6,237,525	132,171	101,807	19,754	1,666,629	23,889	3591
*paps2 paps4*	Rep1	9,888,652	7,388,228	87,001	111,949	17,583	1,505,471	24,218	2189
*paps2 paps4*	Rep2	6,751,835	6,359,793	68,719	72,643	6940	1,750,847	22,807	1440
*bdr1 bdr2 bdr3*	Rep1	13,865,159	13,255,335	152,231	234,740	54,000	1,554,890	25,484	4178
*bdr1 bdr2 bdr3*	Rep2	9,430,105	8,235,268	90,007	104,932	25,957	1,399,030	24,434	2312
*pcfs4*	Rep1	7,944,768	7,571,659	251,258	122,229	37,950	1,490,802	25,800	6593
*pcfs4*	Rep2	5,002,517	4,645,483	130,857	66,422	10,895	1,125,524	22,320	3590
*ssu72*	Rep1	16,427,838	15,800,886	166,448	236,803	37,272	4,088,455	25,326	4070
*ssu72*	Rep2	12,527,314	10,915,776	154,275	180,045	50,414	1,927,984	25,274	4,545
WT (C24)	Rep1	8,634,138	7,927,481	84,512	97,876	14,279	1,830,814	24,747	1892
WT (C24)	Rep2	9,543,267	7,734,138	108,679	84,914	18,792	1,202,444	24,877	2968
*cpsf100*^a	Rep1	12,865,897	12,189,816	266,786	148,034	22,739	3,550,895	24,458	6367
*cpsf100*^a	Rep2	6,459,819	3,753,848	54,402	33,555	4924	1,446,703	22,084	1409
WT (aerial parts)	Rep1	12,129,879	9,157,860	59,993	57,404	8659	1,291,199	21,774	1987
WT (aerial parts)	Rep2	14,259,677	8,971,942	83,569	85,693	8959	2,546,502	23,100	1823
*cstf64*^b	Rep1	9,710,253	8,654,943	135,661	100,624	22,134	2,227,887	21,896	2976
*cstf64*^b	Rep2	6,246,683	5,583,021	88,467	36,860	7257	1,419,914	20,904	2518
*cstf77*^b	Rep1	9,038,164	8,317,489	161,189	80,311	18,451	1,783,396	21,238	3523
*cstf77*^b	Rep2	8,570,612	7,451,354	162,789	71,232	17,983	2,183,317	21,960	4242

Sequencing summary on the FLEP-seq libraries in mutants and corresponding WT.
^aIndicates the use of the C24 background.
^bIndicates the use of the aerial parts of 4-week-old plants.

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Table 1 Summary of FLEP-seq sequencing libraries

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