Fig. 2: GCK binds to importin α1 for nuclear translocation. | Nature Communications

Fig. 2: GCK binds to importin α1 for nuclear translocation.

From: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth

Fig. 2

a MCF7 cells were treated with or without hypoxia for 8 h. Immunoprecipitation with an anti-GCK antibody was performed. b MCF7 cells with or without importin α1 (encoded by KPNA2) depletion were treated with or without hypoxia for 8 h. WCL and cytoplasm and nucleus were prepared. c MCF7 cells expressing the indicated Flag-GCK proteins were treated with or without hypoxia for 8 h. IP with an anti-Flag antibody was performed. d MCF7 and BT549 cells expressing the indicated Flag-GCK proteins were treated with or without hypoxia for 8 h. WCL and cytoplasm and nucleus were prepared. e The indicated Flag-GCK proteins were expressed in MCF7 and BT549 cells. Immunofluorescence analyses were performed with an anti-Flag antibody. DAPI (blue) was used to stain the nuclei. All immunoblotting and immunofluorescence experiments were performed in at least three independent biological replicates, with consistent results observed across repetitions. Representative data are shown. Source data are provided as a Source Data file.

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