Fig. 5: Nuclear GCK-mediated TAZ phosphorylation and activation promotes tumour cell proliferation and tumour growth. | Nature Communications

Fig. 5: Nuclear GCK-mediated TAZ phosphorylation and activation promotes tumour cell proliferation and tumour growth.

From: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth

Fig. 5

a Proliferation of MCF7 and BT549 cells with knocked-in expression of TAZ T346A and the indicated proteins was examined. The data are presented as the means ± SD, n = 3 independent experiments, two-tailed Student’s t test. b Parental MCF7 and BT549 cells and the indicated clones with knock-in expression of TAZ T346A were transfected with the indicated plasmids. The colony formation ability of the MCF7 and BT549 cells was assessed. The data are presented as the means ± SD, n = 3 independent experiments, two-tailed Student’s t test. c, d The parental MCF7 cells and the indicated clones with knock-in expression of GCK S398D or GCK S398A mutants were orthotopically injected into the fourth mammary fat pad of female BALB/c nude mice. The mice were sacrificed 30 days after cell injection. Tumour volume was calculated (n = 5 mice per group). Tumour weights were calculated (n = 5 mice per group). The data are presented as the means ± SDs; two-tailed Student’s t test. e IHC analyses of mouse mammary tumours were performed with the indicated antibodies. Representative staining images are shown. f, g The parental MCF7 cells and the indicated clones with knock-in expression of TAZ T346E or TAZ T346A mutants were orthotopically injected into the fourth mammary fat pad of female BALB/c nude mice. The mice were sacrificed 30 days after cell injection. Tumour volume was calculated (n = 5 mice per group). Tumour weights were calculated (n = 5 mice per group). The data are presented as the means ± SDs; two-tailed Student’s t test. h IHC analyses of mouse mammary tumours were performed with the indicated antibodies. Representative staining images are shown. i, j Parental MCF7 cells and the indicated clones with knock-in expression of TAZ T346A mutants were transfected with WT GCK or GCK S398D and orthotopically injected into the fourth mammary fat pad of female BALB/c nude mice. The mice were sacrificed 30 days after cell injection. The tumour volume was calculated (n = 5 mice per group). Tumour weights were calculated (n = 5 mice per group). The data are presented as the means ± SDs; one-way ANOVA. k IHC analyses of mouse mammary tumours were performed with the indicated antibodies. Representative staining images are shown. Source data are provided as a Source Data file.

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