Fig. 1: Shorter invasion times produced only vacuolar P. aeruginosa.
From: Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
A Representative widefield microscopy images of human corneal epithelial cells (hTCEpi) infected with P. aeruginosa wildtype (WT) at MOI 10 with different invasion times. Images were taken 10 h post-infection at ×40 magnification. P. aeruginosa (green), Hoechst (gray). Scale bar equals 50 µm. B–D Image analysis of timelapse images measuring vacuole numbers (B), percent infected cells (C), and cell death rates (D). Data of biological replicates represented as mean ± SEM, N = 3. For statistical analysis, a Two-way ANOVA with multiple comparisons was performed. Exact P values—A: 7 h = 0.0025, 8h-10h = >0.0001, 11 h = 0.0006, 12 h = 0.0005; B: 0.0025; C: 0.0001. P ≤ 0.01 = **, P ≤ 0.001 = ***, P ≤ 0.0001 = ****. Source data are provided as a Source Data file.
