Fig. 5: Triggering of vacuolar escape depended on T3SS translocon pore proteins.
From: Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
A, B, E, F Image analysis of timelapse images measuring vacuole numbers for wild type (WT) (A, E) or ∆exsA (B, F) in human corneal epithelial cells (hTCEpi) at MOI 50 when co-infected with different strains. C, D, G, H Area Under the Curve bar plots for the percent cells infected with WT (C, G) or ∆exsA (D, H) in presence of other strains. Data of biological replicates represented as mean ± SEM (A, B, E, F) or SD (C, D, G, H), N = 4. For statistical analysis, a Two-way ANOVA with multiple comparisons (A, B, E, F) or a One-way ANOVA with multiple comparisons (C, D, G, H) was performed. Exact P values—A: No co-infection vs. ∆exsE = 0.0083, No co-infection vs. ∆exsE∆STY = 0.0436, ∆exsE vs. ∆exsE∆popBD = 0.0395, ∆exsE vs. ∆exsE∆pscC = 0.0358; B: No co-infection vs. ∆exsE = 0.0402, No co-infection vs. ∆exsE∆STY = 0.0314. P ≤ 0.05 = *, P ≤ 0.01 = **. Source data are provided as a Source Data file.
