Fig. 3: Thor detects fibrotic regions in multiple human heart tissues with MI.

a H&E staining images of tissues from a remote zone (RZ1), an ischemic zone (IZ1), and a fibrotic zone (FZ1). Purple and green squares mark curated ROIs and are annotated as fibrotic and non-fibrotic regions, respectively. Close-up views of the cell morphology and inferred cellular expression of the fibroblast marker gene PDGFRA are provided for the curated ROIs. b Mjolnir-annotated fibrotic regions (blue) are visualized on the H&E staining images. c Bar plot of the percentages of the fibrotic regions in all six samples. d Heatmap of the GO pathway enrichment based on the up-regulated DEGs (fold change >2, adjusted p-value < 0.01 using two-sided Welch’s t-test) in the fibrotic region compared to the non-fibrotic region in each sample, and the up-regulated DEGs (fold change >2, adjusted p-value < 0.01 using two-sided Welch’s t-test) in the non-fibrotic region compared to the fibrotic region in each sample. e TF activity is inferred from the in silico cell spatial transcriptome. We used RTN (R package) for the transcriptional network inference and Cytoscape for network visualization. Source data are provided in a Source Data file.