Fig. 1: MacroH2A1.1 defines TOP1 permissive chromatin domains.
From: Epigenetic control of topoisomerase 1 activity presents a cancer vulnerability
a Heatmaps and profile plots for FLAG-macroH2A1.1 CUT&RUN signal centered on TSS-proximal (TSS) and TSS-distal (non-TSS) TOP1 peaks in MDA-MB-231 macroH2A1.1 KO cells reconstituted with FLAG-macroH2A1.1. Cells without FLAG-macroH2A1.1 served as a negative control (no FLAG), a representative CUT&RUN experiment is shown. b IGV browser shots of distinct macroH2A1.1 chromatin environments associated with TOP1 domains (left) or facultative heterochromatin (HC) marked by H3K27me3 (right). c Jaccard indices for observed peak overlap between a feature of interest (top) and a reference feature (bottom), or randomly shuffled reference feature peaks of equal size (permuted). Values on the y-axis represent the intersection divided by the union in base pairs. d Western blot for the indicated proteins in nuclear lysates (input) or anti-FLAG IP samples from parental and FLAG-macroH2A1.1 knock-in 293 cells (F-1.1) in the presence or absence of CPT and the PARPi Olaparib. Similar results were obtained in a second, independent experiment. * ubiquitinated macroH2A1. Source data are provided as a Source Data file.
