Fig. 3: TOP1cc clearance depends on the macroH2A1.1 PAR binding domain.
From: Epigenetic control of topoisomerase 1 activity presents a cancer vulnerability
a TOP1 RADAR assay with genomic DNA from macroH2A1.1 knockout (1.1KO) MDA-MB-231 cells (–) and 1.1KO cells reconstituted with WT (+1.1WT) or G224E mutant FLAG-macroH2A1.1 (+1.1GE), prior to and at the indicated timepoints after CPT treatment as in Fig. 2f, a representative of three independent experiments is shown. b Quantification of RADAR analysis in (a), depicting the percentage of remaining TOP1cc relative to 0’ after CPT treatment (n = 3 independent replicates), values are expressed as mean and SD. P values are based on two-sided Student’s t-test. Source data are provided as a Source Data file.
