Fig. 6: macroH2A1.1 protects from CPT-induced DNA damage.

a Alkaline comet assay in WT and 1.1 KO MCF7 cells treated with 1 µM CPT or DMSO for 1 h; y-axis depicts Olive tail moment (n > 50 nuclei per sample, see source data for exact n), a representative of two independent experiments is shown, scale bar: 100 µm. b γH2AX IF in EdU-negative (EdU^–) macroH2A1.1 KO (1.1 KO) MCF7 cells reconstituted with empty vector (EV), FLAG-macroH2A1.1 (1.1WT) or FLAG-macroH2A1.1 G224E (1.1GE). Cells were treated with CPT (1 µM, 30 min) with or without 2 h release. Representative EdU^– nuclei are shown, scale bar: 10 µm. Violin plots depict γH2AX foci per nucleus (n > 150 nuclei per sample, see source data for exact n). For all violin plots, center lines (red) reflect the median, white dotted lines depict upper and lower quartiles, p values are based on two-sided Mann-Whitney U test. One of two independent experiments is shown. See Supplementary Fig. 7 for EdU⁺ cells. Source data are provided as a Source Data file.