Fig. 1: Discovery of a novel bacteriocin AMP and BGC. | Nature Communications

Fig. 1: Discovery of a novel bacteriocin AMP and BGC.

From: Antimicrobial peptide class that forms discrete β-barrel stable pores anchored by transmembrane helices

Fig. 1

a Antimicrobial activity indicated by a zone of clearance is restricted to the CT545 S. aureus cluster. The sensitive indicator strain Micrococcus luteus (below-left) is embedded in agar and the test strains (above) spotted. b Plasmid maps of the native pCT545 (NZ_LFNJ01000005.1) and the engineered plasmids pCURE and pRB + BGC for pCT545 replacement (pCT545::pCURE) and BGC complementation, respectively. Outside arcs: plasmid replication and toxin-antitoxin system included in the pCURE plasmid (green) and BGC included in the complementation pRB+BGC plasmid (blue). Inside arcs: for BGC coloring, see Fig. 3b; toxin/antitoxin, black rectangles; putative phage mobilization, grey; replication initiator, white; antibiotic resistance markers, pink. c The pCT545 BGC is necessary for antimicrobial activity and d an immunity mechanism is encoded by pCT545. e Monoculture growth measured by optical density at 600 nm (OD600) of G1905 and USA300 strains expressing Venus or mCerulean fluorescent proteins. f Co-culture growth of G1905 or G1905 pCT545::pCURE expressing mCerulean vs USA300 expressing Venus. Left: mCerulean fluorescence; right: Venus fluorescence. g The reciprocal of f, in which USA300 expresses mCerulean fluorescence and G1905 or G1905 pCT545::pCURE express Venus fluorescence. f, e, g n = 3 biological replicates, mean ± standard error. Images of indicator plates are representative of three independent experiments. Source data are provided as a Source Data file.

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