Fig. 4: S. aureus surfactant peptides boost solubilization of TMcin-G1905. | Nature Communications

Fig. 4: S. aureus surfactant peptides boost solubilization of TMcin-G1905.

From: Antimicrobial peptide class that forms discrete β-barrel stable pores anchored by transmembrane helices

Fig. 4

a Antimicrobial indicator assays of G1905 and G1905 Δagr. b G1905 and G1905 Δagr secreted TMcin-G1905 in culture supernatants or after washing cell pellets with 8 M urea. c Antimicrobial indicator assay demonstrating synergistic activity between TMcin-G1905 and individual S. aureus PSMs. Wells containing TMcin-G1905 were mixed with (+) or without (−) the indicated PSM. d Antimicrobial indicator plate demonstrating a pre-mixing requirement for TMcin-G1905 synergism with δ-toxin, suggesting that PSMs aid in solubilizing TMcin-G1905 to enable it to diffuse through the agar matrix. e Superdex 200 size-exclusion chromatography of G1905 and G1905 Δagr secretions into culture filtrates. Fractions were analyzed by western blot using αTMcin-G1905 rabbit antisera. Images are representative of two (western blots) or three (indicator plates) independent experiments. Source data are provided as a Source Data file.

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