Fig. 1: RNA circularization via PIET and CIRC.
From: Self-splicing RNA circularization facilitated by intact group I and II introns
a Diagram of PIE method for RNA circularization. b Schematic of the PIET approach for RNA circularization, showing the two components as intermediate and 5’-intron. c Assessment of 5’-intron, GTP, and Mg2+ requirements for PIET. d PIET method schematic comparing RNA circularization using different ratios of 5’ intron to intermediate against PIE. e Illustration of CIRC method for RNA circularization. f Agarose gel results depicting GTP and Mg2+ requirements for CIRC. An impaired group I intron was generated by substituting the first 20 bases of the wild-type group I intron with random sequences. g Agarose gel results displaying RNase R-treated linear precursor and circRNA. h Agarose gel showing linear precursor and circRNA treated with poly(A) polymerase. i FACS results illustrating EGFP expression levels in transfected cells. Data presented as mean ± S.D. (n = 3), with each point representing a biological replicate. An unpaired, two-sided Student’s t-test was used for comparisons. j Cell images taken 24 hr post-transfection with linear precursor or circRNA. The linear RNA in this figure was produced using an impaired group I intron. Source data are provided as a Source Data file.
