Fig. 3: Use of additional self-splicing introns in CIRC for RNA circularization.
From: Self-splicing RNA circularization facilitated by intact group I and II introns
a Diagram of the CIRC construct incorporating either group I or group II introns. b Agarose gel electrophoresis showing group I intron splicing and the generation of putative circRNA. c Agarose gel electrophoresis showing group II intron splicing and the generation of putative circRNA. d Agarose gel electrophoresis of putative circRNA generated by different group I introns following treatment with poly(A) polymerase. e Left: Quantification of gel data showing circularization efficiency for various introns. Right: FACS results indicating EGFP expression levels. Data are presented as mean ± S.D. (n = 3), with each point representing a biological replicate. Source data are provided as a Source Data file.
