Fig. 4: Comparison between CIRC and PIE. | Nature Communications

Fig. 4: Comparison between CIRC and PIE.

From: Self-splicing RNA circularization facilitated by intact group I and II introns

Fig. 4

a Quantified circularization efficiency of RNA in CIRC and PIE from agarose gel electrophoresis data. b Comparison of the recovery rate for the final purified circRNA of the correct size. c, d Comparison of Mg2+ (c) and pH (d) requirements. e, f Comparison of circularization rates at 55°C (e) and during IVT reactions at 37°C (f). Data are shown as mean ± S.D. (n = 3), with each symbol representing a biological replicate. Source data are provided as a Source Data file.

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