Fig. 5: Payload size limitations of CIRC.
From: Self-splicing RNA circularization facilitated by intact group I and II introns
a Diagram illustrating payload types and sizes, with cited payloads from previous studies included. b Agarose gel electrophoresis showing spliced intron and RNA integrity of putative circRNA with varying payloads. c Qualitative validation of circRNA using RT-qPCR, since comparisons of the relative abundance of circRNA junctions across different payloads were not feasible. d−h Detection of protein expression for respective payloads: Gluc (d) and Fluc (f) detected with a microplate reader, EGFP (e) detected by FACS, and ABE8e (g) and dystrophin (h) detected by Western blot. Data are shown as mean ± S.D. (n = 3), with each symbol representing a biological replicate. The linear RNA shown in this figure was produced using a modified group I intron with reduced activity. Source data are provided as a Source Data file.
