Fig. 6: Creation of scarless circRNA and immunogenicity testing.
From: Self-splicing RNA circularization facilitated by intact group I and II introns
a Diagram illustrating the principle of generating scarless circRNA using CIRC. b Minimal exon sequence requirements when using the Ana intron in CIRC. c Diagram showing the programmable P1 stem within the Ana intron in CIRC. d Quantified circularization efficiency from agarose gel electrophoresis data for constructs shown in (c). e Diagram illustrating the approach to generate scarless circRNA by using an internal structure as the homology arm. f Quantified circularization efficiency from agarose gel electrophoresis data for scarless circRNA generation using various split sites within CVB3 IRES in CIRC. g A diagram illustrating the workflow for the detection of circRNA immunogenicity, created in BioRender. Shen, Y. (2025) https://BioRender.com/j5i3pnq. h–j qPCR results showing the relative expression of RIG-I, IL-6, and TNF-α following RNA transfection into A549 cells, serving as indicators of immunogenicity. Data are shown as mean ± S.D. (n = 3), with each symbol representing a biological replicate. Source data are provided as a Source Data file.
