Fig. 7: RNase R or Oligo(dT) beads-mediated circRNA purification in CIRC.
From: Self-splicing RNA circularization facilitated by intact group I and II introns
a Diagram depicting RNase R sensitivity of precursors in CIRC and PIE. b Agarose gel electrophoresis showing RNase R treatment of precursors in CIRC and PIE over different time periods. c Agarose-gel electrophoresis showing RNase R treatment of the 2-hr and 4-hr IVT RNA product in CIRC over varying durations. d Top: Diagram illustrating poly(A) insertion within the intron in CIRC. Bottom: Workflow for oligo(dT) column-mediated circRNA purification. e Agarose gel electrophoresis showing the effect of different number of As insertion on circularization (f) Agarose gel electrophoresis of the flow-through from the oligo(dT) column. In (b−f) no additional replicate was done. Source data are provided as a Source Data file.
