Table 1 Reaction conditions for additional RNA circularization
From: Self-splicing RNA circularization facilitated by intact group I and II introns
Samples in Figures | Concentration of MgCl2 (mM) | Buffer | pH | Temperature | Reaction time (min) | Concentration of NaCl (mM) |
---|---|---|---|---|---|---|
Fig. 1c, d and f | 10 mM | 50 mM HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane sulfonic acid) | 6.8 | 55°C | 60 | 150 |
Fig. 2 | 6 mM | 50 mM HEPES | 6.8 | 37°C or 55°C | 60 | \ |
Fig. 3 | 6 mM | 50 mM HEPES | 6.8 | 55°C | 60 | \ |
Fig. 4c | As shown in the figure | \ | \ | 55°C | 60 | \ |
Fig. 4d | 10 mM | As shown in the figure | As shown in the figure | 55°C | 60 | \ |
Fig. 4e | As shown in the figure | 50 mM MES | 6.5 | 55°C | 60 | \ |
Fig. 5b | 4 mM or 6 mM | 50 mM MES | 6.0 or 6.5 | 55°C | 15 | \ |
Fig. 6b, d and f | 6 mM | 50 mM HEPES | 6.8 | 55°C | 60 | \ |
Fig. 7e | 6 mM | 50 mM HEPES | 6.8 | 55°C | 60 | \ |