Table 1 Reaction conditions for additional RNA circularization

From: Self-splicing RNA circularization facilitated by intact group I and II introns

Samples in Figures

Concentration of MgCl2 (mM)

Buffer

pH

Temperature

Reaction time (min)

Concentration of NaCl (mM)

Fig. 1c, d and f

10 mM

50 mM HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane sulfonic acid)

6.8

55°C

60

150

Fig. 2

6 mM

50 mM HEPES

6.8

37°C or 55°C

60

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Fig. 3

6 mM

50 mM HEPES

6.8

55°C

60

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Fig. 4c

As shown in the figure

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55°C

60

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Fig. 4d

10 mM

As shown in the figure

As shown in the figure

55°C

60

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Fig. 4e

As shown in the figure

50 mM MES

6.5

55°C

60

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Fig. 5b

4 mM or 6 mM

50 mM MES

6.0 or 6.5

55°C

15

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Fig. 6b, d and f

6 mM

50 mM HEPES

6.8

55°C

60

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Fig. 7e

6 mM

50 mM HEPES

6.8

55°C

60

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