Table 1 Statistical evaluation of synergy using the stringent fractional product as an additivity reference identifies two drug combinations that show synergy over multiple time points

From: Challenges to determine synergistic drug interactions in mice

  1. Synergy thresholds are displayed, where the red color indicates that the synergy threshold has been reached. The p-value according to Webb17 assesses the difference of measured versus fractional product using a t-test, two-sided, based on log-transformed luminescence data of the measured effect of a combination versus the expected additive effect based on the fractional product. ND indicates that the normal distribution could not be assessed due to the absence of mice in the control group at that particular time point. Normal distribution was assessed using a Jarque–Bera test. NA indicates that synergy could not be calculated (for Tagrisso, AZD2014, and DOC) or was not calculated because all other assessments were negative (Mitomycin C and A443654). For the combination Imatinib and Dasatinib, an error (fraction versus percentage) was present in the original publication, ruling out synergy at one time point rather than the originally reported two time points which are now re-assessed.
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