Fig. 1: Generation of tumoroid models from EwS and CDS tumors.
From: Patient-derived tumoroids from CIC::DUX4 rearranged sarcoma identify MCL1 as a therapeutic target
a Scheme depicting the drug profiling pipeline with separate arms for direct drug profiling (lower arm) and profiling after generation of ex vivo tumoroid models (upper arm). Created in BioRender. Wachtel, M. (2025) https://BioRender.com/t5q1jju. b Phase-contrast microscopy images illustrating the development of three EwS models. Images were taken at an early phase of culture in Matrigel domes (left panels) and at later time points after cell expansion in Matrigel domes or on a thick layer of Matrigel (middle and right panels, respectively). Each tumoroid was established once from human tumor material. Scale bar, 400 μm. c Time required to generate individual tumoroid models (left panels) and overall success rate (right panels) for EwS and CDS tumors. d Viability of indicated EwS and CDS cells after treatment with bFGF, EGF and IGF1 for seven days relative to cells grown in absence of growth factors. Cell viability was assessed using CTG assay. Plotted are means ± SD. n = 4 independent experiments, two-way ANOVA, Tukey’s multiple comparisons test. Source data are provided as a Source Data file.
