Fig. 4: MCL1 inhibition efficiently induces apoptosis in CDS tumor cells.

a Dose-response curves for CDS (left panel) and EwS (right panel) tumoroid models treated with the MCL1 inhibitor S64315. Cell viability was measured at the three indicated time points by CTG assay. Data are presented as means. n = 2 independent experiments. b IC50 values calculated from the data shown in a after 72 h of treatment with S64315. c Detection of cell death after treatment of CDS-ZH003 with the indicated doses of S64315 for 24 h using Annexin V and 7-AAD staining followed by flow cytometry. Representative pseudocolor density plots illustrate the number of live and dead cells (upper and lower-left panels). The percentage of dead cells, defined as Annexin V and/or 7-AAD positive, is shown in the lower right panel. Plotted are means ± SD. n = 3 independent experiments, one-way ANOVA, Tukey’s multiple comparisons test. d Western blot analysis of cell lysates from three indicated CDS tumoroid models after treatment with S64315 for 24 h. n = 1 independent experiment. e Viability of CDS-ZH003 cells after treatment with the indicated dose range of S64315 in presence or absence of different doses of Z-VAD-FMK. Plotted are means ± SD. n = 3 independent experiments, two-way ANOVA, Tukey’s multiple comparisons test. Source data are provided as a Source Data file.