Fig. 1: Phenotypes of OAP1, OAP2, CDC48D and CIP111 mutants.

A Developing seeds in siliques collected from Col-0 control plants, oap1, oap2, cdc48D and cip111 mutants, along with the complemented mutants and mutants crossed with Col-0 plants. White arrows indicate aborted ovules or seeds. ap1; OAP1-mNG is oap1 mutant complemented with pOAP1:OAP1-mNeonGreen. oap2; pOAP2:OAP2 is oap2 mutant complemented with pOAP2:OAP2. cip111; mScar-CIP111m is cip111 mutant complemented with pCIP111:mScarlet-CIP111m. cdc48D; mScar-CDC48D is cdc48D mutant complemented with pCDC48D:mScarlet-CDC48D. B Ovules from Col-0 control plants, oap1, oap2, cdc48D, and cip111 mutant plants. White arrows indicate the nucleolus. ccn: central cell nucleolus, ecn: egg cell nucleolus, scn: synergid cell nucleolus. C Zygotes from Col-0 control plants, opnr, oap1 and oap2 mutant plants. Red arrows indicate the vacuole, while white arrows indicate the nucleolus. Scale bars: A 0.5 mm; B, C 10 μm.