Fig. 1: Light-induced actin polymerization in live cells. | Nature Communications

Fig. 1: Light-induced actin polymerization in live cells.

From: Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins

Fig. 1

a Comparison of the regulatory mechanisms of WRC (left) and OptoVCA system (right). b Representative images of MDCK cells simultaneously expressing Stargazin-mEGFP-iLID and Lifeact-miRFP703 (right panels) along with SspB-mScarlet-I (upper left) or SspB-mScarlet-I-VCA (lower left). For each cell, protein localizations before (upper) and after (lower) blue light illumination are shown. Normalized translocation levels of SspB-mScarlet-I (c, Control) or SspB-mScarlet-I-VCA (c, OptoVCA) and Lifeact-miRFP703 (d) to the cortex. The mean values (bold lines) are plotted as a function of time with the SD. n = 37 for each cell. e Relationship between the normalized translocation levels of SspB-mScarlet-I-VCA and Lifeact-miRFP703, and the expression level of SspB-mScarlet-I-VCA. The translocation levels of SspB and Lifeact are defined as the mean values of SspB-mScarlet-I-VCA and Lifeact-miRFP703 at t = 2–8 min in (c, d), respectively. n = 37 cells. f Representative images of the cells expressing Stargazin-mEGFP-iLID, SspB-mScarlet-I-VCA, and Lifeact-miRFP703 treated with DMSO (left) or CK-666 (right) before (upper) and after (lower) blue light illumination. g Normalized cortical fluorescence intensity of Lifeact-miRFP703 in (f). The mean values (bold lines) are plotted as a function of time with the SD. n = 18 and 17 for DMSO and CK-666 treated cells, respectively. All scale bars, 20 μm. p values were calculated by unpaired two-sided t-test at t = 5 min. ***p < 0.001. n.s. p  ≥ 0.05. Source data are provided as a Source Data file.

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