Fig. 4: Control of actin density by light intensity. | Nature Communications

Fig. 4: Control of actin density by light intensity.

From: Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins

Fig. 4

a Representative images of the recruitment of SspB-mScarlet-I-VCA (upper panel) and subsequent actin polymerization (lower panels) under five different light-powered illuminations on 6% DGS-NTA(Ni) lipid. The bottom plane, top plane, and three-dimensional (3D) reconstructed slice images at each indicated time point are shown. Condition: 5 μM Actin, 15 μM Profilin, 100 nM Arp2/3 complex, 25 nM CP, and 150 nM SspB-mScarlet-I-VCA. Blue squares indicate the illuminated regions. All scale bars, 20 μm. b, c Fluorescence intensities of SspB-mScarlet-I-VCA under the five different light-powered illuminations. The extreme outliers in (c) are attributed to focus drift of the microscope. d, e Fluorescence intensities of Alexa647-actin with five different light-powered illuminations. b, d The mean values (bold lines) are plotted as a function of time with the SD. n = 6 for each condition. For box plots in (c, e), the mean fluorescence intensities of SspB-mScarlet-I-VCA in (b) and Alexa647-actin in (d) at t = 11-20 min are used, respectively. n = 6 for each condition. p values were calculated by one-way ANOVA followed by Tukey’s multiple comparisons test. ***p < 0.001. Source data are provided as a Source Data file.

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