Fig. 1: Experimental design, unsupervised clustering, and sub-clustering of human α cells by scRNA-seq and snRNA-seq of islets from adult human non-diabetic donors. | Nature Communications

Fig. 1: Experimental design, unsupervised clustering, and sub-clustering of human α cells by scRNA-seq and snRNA-seq of islets from adult human non-diabetic donors.

From: Human pancreatic α-cell heterogeneity and trajectory inference analyses reveal SMOC1 as a β-cell dedifferentiation gene

Fig. 1

a Human islet processing and data generation scheme. Created in BioRender. Garcia-Ocana, A. (2025) https://BioRender.com/nizfh1l. b Unsupervised clustering, cell type annotated UMAP and separated α-cell cluster (below). c. Pre-annotated α-cell subclusters by assigning Louvain resolution 0.8. d. Annotated α-cell clusters according to the UMAP location relative to neighboring β-cells17 and gene expression. UMAP is split by the processing type – scRNA-seq (left) and snRNA-seq (right). e Proportion of the α-cell subtypes in scRNA-seq and snRNA-seq datasets.

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