Fig. 4: Trajectories between α-cells and β-cells and their associated gene expression pattern. | Nature Communications

Fig. 4: Trajectories between α-cells and β-cells and their associated gene expression pattern.

From: Human pancreatic α-cell heterogeneity and trajectory inference analyses reveal SMOC1 as a β-cell dedifferentiation gene

Fig. 4

a Expression of genes with selective high association with Trajectory A (from AB to β1). Genes were clustered according to the expression pattern. The visualization is done by Monocle3, by separating the relevant clusters and assigning the root according to the PAGA analysis. b Expression of genes with selective high association with Trajectory B (β3 to α2). c Expression of genes with selective high association with Trajectory C (AB to α2). d Expression of genes with selective high association with Trajectory D (α3 to α2). e Gene commonality analysis towards α-cell transition. Genes in the intersection of Trajectories B, C, and D revealed 10 common genes among the different trajectories. f Dot plots representing gene expression of the commonality genes between Trajectories B, C and D in scRNA-seq data (top) and snRNA-seq data (bottom). g Trajectories between α-cells and β-cells and their associated gene expression pattern. Enrichment ridge plot of GOBP’s pancreatic α-cell differentiation pathway for the common genes among the Trajectories B, C and D. We grouped α-cells in two groups by expression level for each gene – negative (0% normalized expression), positive (>0%).

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