Fig. 3: Deficiency of macrophage TIPE2 promotes ASC ferroptosis in VAT of HFD-fed mice. | Nature Communications

Fig. 3: Deficiency of macrophage TIPE2 promotes ASC ferroptosis in VAT of HFD-fed mice.

From: Obesity-associated macrophages dictate adipose stem cell ferroptosis and visceral fat dysfunction by propagating mitochondrial fragmentation

Fig. 3

a, b Representative images of H&E staining (a) and statistical analysis of adipocyte sizes (b; n ≥ 395 adipocytes examined over 3 mice per group) in VAT sections from mice indicated in Fig. 2. Scale bars, 50 μm. c–f Heat map (c) for adipogenesis gene expression, PPAR-γ mRNA (d) and protein (e, f) levels in epididymal VAT. g, h Representative confocal imaging of immunofluorescence for 4-HNE (green) in ASCs (Sca-1, red) (g) and ACSL4 (red) in ASCs (Sca-1, green) (h) in VAT sections. Scale bars, 2 μm (g) and 5 μm (h). i–n Flow cytometry frequencies of ASCs (Sca-1+CD45-) in SVF (i, j), cellular death (i, k) and mitochondrial ROS (l, m) in ASCs with MFI quantification (n) in VAT. n = 3 mice per group (c, d, f, j, k, m, n). Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-way ANOVA (b, f) or two-tailed student’s t-test (d, j, k, m, n).

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