Fig. 6: Tipe2-/- macrophage transfer promotes ASC ferroptosis and metabolic disorders in HFD-fed mice. | Nature Communications

Fig. 6: Tipe2-/- macrophage transfer promotes ASC ferroptosis and metabolic disorders in HFD-fed mice.

From: Obesity-associated macrophages dictate adipose stem cell ferroptosis and visceral fat dysfunction by propagating mitochondrial fragmentation

Fig. 6

a Flow cytometry frequency of Tipe2-/- macrophage-derived mitochondria in ASCs of SVF from HFD-fed wild-type mice received intraperitoneal transfer of Tipe2-/- macrophages (labeled with MitoTracker and treated with FA for 18 h). n = 3 mice per group. b–f Body weight change (b), glucose tolerance test with area under the curve (c, d), insulin tolerance test with area under the curve (e, f) in mice received intraperitoneal transfer of Tipe2+/+ or Tipe2-/- macrophages (18 h pretreatment with FA) during the last 4 weeks of HFD feeding (7 weeks). 2 × 106 macrophages per mice, every 3 days. g, h Ratios of EpiVAT mass (g), liver weight (h) to body weight. i, Representative confocal imaging of immunofluorescence for 4-HNE (red) in ASCs (Sca-1, green) in VAT sections. Scale bars, 10 μm. j–n Flow cytometry frequencies of ASCs in SVF (j, k), cellular death in ASCs (j, l), mitochondrial ROS in ASCs (j,m) with MFI quantification (n) in VAT. n = 5 mice per group (b), n = 3 or 4 mice per group (c-h, k-n). Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 determined by two-tailed student’s t-test (a, d, f–h, k–n) or two-way ANOVA (c,e).

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