Fig. 2: Pre-granulosa (PG) cells emerge from mesenchymal-like progenitors at W5–6.
From: Defining the cell and molecular origins of the primate ovarian reserve
A, B Spatial distribution of genes of interest in Visium CytAssist analysis of W5 and W6 ovaries mapped onto a black and white H&E image. Ovary (white), mesonephros (orange), and adrenal (yellow) regions are outlined; expression level scaled from blue (min) to yellow (max). C CosMx spatial molecular imager (SMI) analysis of W6_3 ovaries; left panels, distribution of annotated cell clusters in the selected fields of view (FOVs); right panels, zoom in on ovary region with cell segmentation overlay showing germline, pre-granulosa and stromal cells (additional clusters marked “Others”). White dashed line indicates ovary region. D Immunofluorescence analysis for known genes (WT1, blue; GATA4, orange; FOXL2, yellow; VASA, green; NR2F2, magenta) and genes identified in the DEG analysis (NR5A1, red; GATM, cyan) in W5 (n = 2) and W6 (n = 2) ovaries. Scale bars 50 μM.
