Fig. 1: Pupillary constriction’s predicted monocular and binocular responses in RGC axons in vivo, compared to canonical responses.
From: Pupil size modulation drives retinal activity in mice and shapes human perception
A Schematic of retinal AAV injection to label RGC axons in the dLGN (green) for in vivo 2-photon imaging. B RGC axons in the dLGN (C), RGC boutons during 2-photon imaging (representative of N = 12 animals). D Schematic of possible OFF cell responses: an OFF cell where pupillary constriction plays no role in the response, it only shows a canonical response at light offset to stimulus presented to the injected eye, from the screen contralateral to the recording site (left), an OFF cell exhibiting a predicted pupil-induced response from contralateral screen stimulus (middle, red arrow), an OFF cell exhibiting a predicted pupil-induced binocular response to stimulating the non-injected eye from ipsilateral screen stimulus (right, red arrowhead). E Example RGC activity showing canonical OFF response to contralateral eye stimulation (left, purple) and no binocular response to ipsilateral eye stimulation (right, green), contralateral pupil responses to each stimulus in bottom. Data are presented as mean ± SEM. F Example RGC showing a response consistent with predicted pupil-induced monocular response to contralateral eye stimulation (left, purple, red arrow), as well as a binocular response to ipsilateral eye stimulation (right, green, red arrowhead), contralateral pupil responses to each stimulus in bottom. Data are presented as mean ± SEM. All flashes are from a 9.5 Lux baseline to 31 Lux.
