Fig. 2: Binocular RGC activity is due to pupillary constriction.
From: Pupil size modulation drives retinal activity in mice and shapes human perception
A RGC responses to full-field flash stimulus to the contralateral (top, purple) or ipsilateral eye (bottom, green) in anesthetized animals, for example response (middle, mean ± SD) and heatmap of individual boutons (right). QI = quality index (see “Methods”) (n = 649 boutons N = 5 animals). Throughout figure, purple indicates contralateral visual stimulation and green ipsilateral stimulation. B Population average of heatmaps in A (mean ± SEM). C Histogram of responses in (A). D Same as (A), but for awake, freely moving RGC responses (n = 709 boutons, N = 4 animals). E Population average of heatmaps in (D). F Histogram of responses in (D). G Pupillary constriction from full-field stimulus (middle) to either contralateral (left) or ipsilateral (right) stimulation (mean ± SEM), and RGC population responses in the same animals (bottom) (mean ± SEM n = 849 boutons, N = 5 animals). H Peak non-canonical OFF responses (normalized, RMI) in anesthetized and awake animals to contralateral or ipsilateral stimulation (n = 649, N = 5 and n = 709, N = 4, respectively). Two-sided two-sample t-test. Violin plot with median labeled as white point. I Spearman correlation between RGC activity and pupillary constriction due to contralateral (top) or ipsilateral (bottom) stimulation, before and after atropine. ***p < 0.001 (two-sample two-sided Kolmogorov–Smirnov test) (n = 849 boutons, N = 5 animals, n = 848 boutons, N = 4 animals, respectively). J Heatmap of RGC axons imaged in G. K RGC responses of boutons in (G–I) after administration of atropine to the contralateral eye, eliminating non-canonical OFF responses due to contralateral stimulation (left) and ipsilateral responses entirely (right). L Heatmap of RGC axons after TTX injection into the ipsilateral eye, seeing no change in contralateral activity, including the non-canonical OFF response (left), but completely eliminating ipsilateral responses (right) (n = 187 boutons, N = 3 animals). (M) Peak non-canonical OFF responses for contralateral (left) or ipsilateral (right) stimulation shown in (I–K). ***p < 0.001 (two-sample two-sided t-test, multiple comparisons corrected with Bonferroni correction). Violin plot with median labeled as white point.
