Fig. 2: Function of TcMscS.
From: Lipid-mediated gating of a miniature mechanosensitive MscS channel from Trypanosoma cruzi
a TcMscS inactivation in the absence of sustained tension. Over time, increased stimulus amplitudes failed to induce channel opening. Insets: opening and closure of TcMscS channels in response to the first − 90 mmHg pressure ramp (left) and activation of endogenous EcMscL channels, but not TcMscS channels at the near-lytic − 150 mmHg pressure ramp (right). Symmetric KCl buffer (200 mM KCl, 90 mM MgCl2, 2 mM CaCl2, and 5 mM HEPES, pH 7.2) was used, and all measurements were carried out on excised inside-out patches at − 30 mV membrane potential. b Channel inactivation under sustained tension (− 60 mmHg pressure step). Subsequent near-lytic − 95 mmHg pressure ramp only induced activation of endogenous EcMscL channels, but not TcMscS channels. cTcMscS C66L showed no significant inactivation either in the absence of tension or under sustained (− 60 mmHg step) tension. d Cryo-EM structure of TcMscS C66L (in gray) and overlay with the wild-type TcMscS (in green) structure.
