Fig. 5: OL dynamics in dentate gyrus, induction of endogenous EPO, and consequence of EPOR deletion in MOL.
a–d Differential OL dynamics in the dentate gyrus upon rhEPO/placebo. a Area of analysis. b Quantification of late OPC/COP/NFOL at 24 h, 3 and 4 weeks following rhEPO/placebo initiation. c Quantification of NFOL at 3 and 4 weeks after start of rhEPO/placebo. d Quantification of MOL following 1 week, 3 weeks and 4 weeks after initiation of rhEPO/placebo. e, f Induction of endogenous EPO by motor cognitive challenge and inspiratory hypoxia. e Experimental design: Starting on P28, mice are exposed to normoxia (21% O2; N), functional hypoxia induced by complex running wheel (CRW) performance, inspiratory hypoxia (12% O2; H), or CRW upon inspiratory hypoxia (H + CRW), for 3 weeks. f Quantification of late OPC/COP/NFOL in CA1 and CA3. g–k Effect of rhEPO in mice with EPOR deletion in MOL. g Experimental design: CnpCre::EPOR mice received rhEPO once (24 h) or for 3 weeks and brains were examined at week 4. h Quantification of OPC and i late OPC/COP/NFOL in CA1 and CA3 at 24 h. j Quantification of late OPC/COP/NFOL and k MOL in CA1 and CA3 at 4 weeks; mean ± SEM; n (number of mice) given inside the bars; unpaired two-tailed Student’s t-test except for i (CA3), where two-tailed Mann-Whitney U test was performed; in f, Tukey’s one-way ANOVA. a, e, g Created in BioRender. ye, l. (2025) https://BioRender.com/s87m1ln. Source data are provided as a Source Data file.
