Fig. 6: Deletion of EPOR in MOL has network-relevant influence on myelination.
a Experimental design using CnpCre::EPOR mice and controls. b Representative images and c quantification of myelin (MBP+ area) in CA1 and CA3. d Representative images with microglia marker IBA1 and phagocytic (activation) marker CD68 and e,f quantification of microglia and activated microglia. g, h Quantification of OPC and MOL in CA1 and CA3 of CnpCre+/-::EPOR+/+ and CnpCre+/-::EPORfl/fl mice. i Representative EM images from fimbria of CnpCre::EPOR mice, showing no appreciable difference between genotypes. j Highly significant right-shift of myelinated axon calibers in fimbria, but no difference in myelin thickness (g-ratio) and myelinated axons/µm2. k Representative EM images from corpus callosum of CnpCre::EPOR mice, revealing no systematic alteration of myelinated axon calibers in fimbria, and no difference in myelin thickness (g-ratio) and myelinated axons/µm2. m Representative EM image from CA1 of CnpCre::Epor mice with n unchanged myelinated axons/µm2. o Comparison of Jacobian determinants in MRI of CnpCre+/-::EPORfl/fl versus CnpCre+/-::EPOR+/+ mice: red/yellow color indicates local tissue volume expansions, blue/cyan local reductions. p Volumetric MRI comparison of fimbria and striatum; scale bars: b, d 50 µm, I, k, m 1 µm; mean ± SEM presented, with n (number of mice) given inside the bars; two-tailed Mann-Whitney U test was performed for n, asymptotic two-sample Fisher-Pitman permutation tests for j and l, unpaired two-tailed Student’s t-tests for the others. a Created in BioRender. ye, l. (2025) https://BioRender.com/s87m1ln. Source data are provided as a Source Data file.
