Fig. 3: Gain of CPDphr function confers enhanced cell survival and reduced levels of DNA damage upon oxidative stress in mammalian cells.

a Violin plot showing quantification of immunofluorescence assay measurements of γ-H2AX levels in 3T3 (grey) and 3T3 CPD cells (blue) treated with a range of H₂O₂ concentrations up to 400 µM indicated on the x-axis. The horizontal black lines within the violin plots indicate mean values and the black hollow circles represent individual data points (n = 9 biologically independent samples). b AHM assay results in 3T3 and 3T3 CPD cells exposed to a range of H₂O₂ concentrations up to 50 µM. Total numbers of dead and viable cells are plotted as means ± SEM (n = 4 biologically independent samples) normalized in relation to untreated cells (Ctrl) on the y-axis, while H₂O₂ concentrations are indicated on the x-axis. c MTT assay results of 3T3 and 3T3 CPD cells exposed to H₂O₂ concentrations ranging from 200 to 3000 µM. Mean percentage ± SEM (n = 8 biologically independent samples) cell viability with respect to untreated cells is plotted on the y-axis, while H₂O₂ concentrations are indicated on the x-axis. Controls confirming that the transfection and subsequent selection procedures are not responsible for the observed results with the 3T3 cells are provided where mutant forms of CPDphr were ectopically expressed in 3T3 cells and results equivalent to those from untransfected 3T3 cells were obtained (see below). All experiments were conducted in constant darkness (DD) and repeated at least 3 times, independently. For the cell viability assays (b, c), representative data is shown. The statistical test used for (a) is student’s t-test, while the analysis for (b, c) is two-way ANOVA analysis. Statistical differences (P values) are indicated in each panel. Source data are provided as a Source Data file.